[HTML][HTML] Optimising the quantification of cytokines present at low concentrations in small human mucosal tissue samples using Luminex assays
E Staples, RJM Ingram, JC Atherton… - Journal of immunological …, 2013 - Elsevier
Sensitive measurement of multiple cytokine profiles from small mucosal tissue biopsies, for
example human gastric biopsies obtained through an endoscope, is technically challenging.
Multiplex methods such as Luminex assays offer an attractive solution but standard
protocols are not available for tissue samples. We assessed the utility of three commercial
Luminex kits (VersaMAP, Bio-Plex and MILLIPLEX) to measure interleukin-17A (IL-17) and
interferon-gamma (IFNγ) concentrations in human gastric biopsies and we optimised …
example human gastric biopsies obtained through an endoscope, is technically challenging.
Multiplex methods such as Luminex assays offer an attractive solution but standard
protocols are not available for tissue samples. We assessed the utility of three commercial
Luminex kits (VersaMAP, Bio-Plex and MILLIPLEX) to measure interleukin-17A (IL-17) and
interferon-gamma (IFNγ) concentrations in human gastric biopsies and we optimised …
