Peripheral macrophage recruitment in cuprizone-induced CNS demyelination despite an intact blood–brain barrier
EJ McMahon, K Suzuki, GK Matsushima - Journal of neuroimmunology, 2002 - Elsevier
The contribution of peripheral macrophage was assessed in cuprizone intoxication, a model
of demyelination and remyelination in which the blood–brain barrier remains intact. Flow
cytometry of brain cells isolated from cuprizone-treated mice revealed an increase in the
percentage of Mac-1+/CD45hi peripheral macrophage. To confirm these results in situ,
C57BL/6 mice were lethally irradiated, transplanted with bone marrow from GFP-transgenic
mice, and exposed to cuprizone. GFP+ peripheral macrophages were seen in the CNS after …
of demyelination and remyelination in which the blood–brain barrier remains intact. Flow
cytometry of brain cells isolated from cuprizone-treated mice revealed an increase in the
percentage of Mac-1+/CD45hi peripheral macrophage. To confirm these results in situ,
C57BL/6 mice were lethally irradiated, transplanted with bone marrow from GFP-transgenic
mice, and exposed to cuprizone. GFP+ peripheral macrophages were seen in the CNS after …