An economical adaptation of the RosetteSep™ procedure for NK cell enrichment from whole blood, and its use with liquid nitrogen stored peripheral blood …

HS Warren, PM Rana - Journal of immunological methods, 2003 - Elsevier
This study reports an economical adaptation of the RosetteSep™ procedure for enrichment
of NK cells designed for whole blood and its use with liquid nitrogen stored peripheral blood
mononuclear cells (PBMC). Combining 45× 106 PBMC with 45× 108 Alsevers' stored red
blood cells (RBC) in 1 ml requires 50 μl of RosetteSep™ bifunctional antibody cocktail and
provides NK cells of 99% purity with average yields of 43%.