[HTML][HTML] N-glycosylation determines the abundance of the transient receptor potential channel TRPP2

A Hofherr, C Wagner, S Fedeles, S Somlo… - Journal of Biological …, 2014 - ASBMB
Glycosylation plays a critical role in the biogenesis and function of membrane proteins.
Transient receptor potential channel TRPP2 is a nonselective cation channel that is mutated
in autosomal dominant polycystic kidney disease. TRPP2 has been shown to be heavily N-
glycosylated, but the glycosylation sites and the biological role of N-linked glycosylation
have not been investigated. Here we show, using a combination of mass spectrometry and
biochemical approaches, that native TRPP2 is glycosylated at five asparagines in the first …