[HTML][HTML] Alternative splicing determines the post-endocytic sorting fate of G-protein-coupled receptors
M Tanowitz, JN Hislop, M von Zastrow - Journal of biological chemistry, 2008 - ASBMB
Mu-type opioid receptors are physiologically important G-protein-coupled receptors that are
generally thought to recycle after agonist-induced endocytosis. Here we show that several
alternatively spliced receptor variants fail to do so efficiently because of splice-mediated
removal of an endocytic sorting sequence that is present specifically in the MOR1 variant. All
of the recycling-impaired receptor variants were found to undergo proteolytic down-
regulation more rapidly than MOR1, irrespective of moderate differences in endocytic rate …
generally thought to recycle after agonist-induced endocytosis. Here we show that several
alternatively spliced receptor variants fail to do so efficiently because of splice-mediated
removal of an endocytic sorting sequence that is present specifically in the MOR1 variant. All
of the recycling-impaired receptor variants were found to undergo proteolytic down-
regulation more rapidly than MOR1, irrespective of moderate differences in endocytic rate …