[HTML][HTML] BCL-2 selectively interacts with the BID-induced open conformer of BAK, inhibiting BAK auto-oligomerization
SC Ruffolo, GC Shore - Journal of Biological Chemistry, 2003 - ASBMB
Caspase-8 cleaves BID to tBID, which targets mitochondria and induces oligomerization of
BAX and BAK within the outer membrane, resulting in release of cytochrome c from the
organelle. Here, we have initiated these steps in isolated mitochondria derived from control
and BCL-2-overexpressing cells using synthetic BH3 peptides and subsequently analyzed
the BCL members by chemical cross-linking. The results show that the BH3 domain of BID
interacts with and induces an" open" conformation of BAK, exposing the BAK N terminus …
BAX and BAK within the outer membrane, resulting in release of cytochrome c from the
organelle. Here, we have initiated these steps in isolated mitochondria derived from control
and BCL-2-overexpressing cells using synthetic BH3 peptides and subsequently analyzed
the BCL members by chemical cross-linking. The results show that the BH3 domain of BID
interacts with and induces an" open" conformation of BAK, exposing the BAK N terminus …
