[HTML][HTML] Design of the artificial acellular feeder layer for the efficient propagation of mouse embryonic stem cells
M Nagaoka, Y Hagiwara, K Takemura… - Journal of biological …, 2008 - ASBMB
Embryonic stem (ES) cells are pluripotent-undifferentiated cells that have a great interest for
the investigation of developmental biology. Murine ES cells maintain their pluripotency by
the supplementation of the leukemia inhibitory factor (LIF). LIF is reported to act as a matrix-
anchored form, and immobilized cytokines are useful to sustain their signaling on target
cells. In this study, we used the immobilizable fusion protein composed of LIF and IgG-Fc
region, which was used as a model of the matrix-anchored form of LIF to establish a novel …
the investigation of developmental biology. Murine ES cells maintain their pluripotency by
the supplementation of the leukemia inhibitory factor (LIF). LIF is reported to act as a matrix-
anchored form, and immobilized cytokines are useful to sustain their signaling on target
cells. In this study, we used the immobilizable fusion protein composed of LIF and IgG-Fc
region, which was used as a model of the matrix-anchored form of LIF to establish a novel …