siRNAs can function as miRNAs
JG Doench, CP Petersen, PA Sharp - Genes & development, 2003 - genesdev.cshlp.org
JG Doench, CP Petersen, PA Sharp
Genes & development, 2003•genesdev.cshlp.orgWith the discovery of RNA interference (RNAi) and related phenomena, new regulatory roles
attributed to RNA continue to emerge. Here we show, in mammalian tissue culture, that a
short interfering RNA (siRNA) can repress expression of a target mRNA with partially
complementary binding sites in its 3′ UTR, much like the demonstrated function of
endogenously encoded microRNAs (miRNAs). The mechanism for this repression is
cooperative, distinct from the catalytic mechanism of mRNA cleavage by siRNAs. The use of …
attributed to RNA continue to emerge. Here we show, in mammalian tissue culture, that a
short interfering RNA (siRNA) can repress expression of a target mRNA with partially
complementary binding sites in its 3′ UTR, much like the demonstrated function of
endogenously encoded microRNAs (miRNAs). The mechanism for this repression is
cooperative, distinct from the catalytic mechanism of mRNA cleavage by siRNAs. The use of …
With the discovery of RNA interference (RNAi) and related phenomena, new regulatory roles attributed to RNA continue to emerge. Here we show, in mammalian tissue culture, that a short interfering RNA (siRNA) can repress expression of a target mRNA with partially complementary binding sites in its 3′ UTR, much like the demonstrated function of endogenously encoded microRNAs (miRNAs). The mechanism for this repression is cooperative, distinct from the catalytic mechanism of mRNA cleavage by siRNAs. The use of siRNAs to study translational repression holds promise for dissecting the sequence and structural determinants and general mechanism of gene repression by miRNAs.
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