Differentiation of new insulin-producing cells is induced by injury in adult pancreatic islets
A Fernandes, LC King, Y Guz, R Stein… - …, 1997 - academic.oup.com
A Fernandes, LC King, Y Guz, R Stein, CVE Wright, G Teitelman
Endocrinology, 1997•academic.oup.comThe ability of the adult pancreas to generate new insulin (β) cells has been controversial
because of difficulties in unequivocally identifying the precursor population. We recently
determined that β cells were generated during development from precursors that expressed
the homeodomain-containing transcription factor pancreas duodenum homeobox gene-1
(PDX-1). To investigate whether PDX-1+ stem cells are present in adult pancreas, we
examined two animal models of diabetes. One model was produced by injecting adult mice …
because of difficulties in unequivocally identifying the precursor population. We recently
determined that β cells were generated during development from precursors that expressed
the homeodomain-containing transcription factor pancreas duodenum homeobox gene-1
(PDX-1). To investigate whether PDX-1+ stem cells are present in adult pancreas, we
examined two animal models of diabetes. One model was produced by injecting adult mice …
Abstract
The ability of the adult pancreas to generate new insulin (β) cells has been controversial because of difficulties in unequivocally identifying the precursor population. We recently determined that β cells were generated during development from precursors that expressed the homeodomain-containing transcription factor pancreas duodenum homeobox gene-1 (PDX-1). To investigate whether PDX-1+ stem cells are present in adult pancreas, we examined two animal models of diabetes. One model was produced by injecting adult mice with streptozotocin (SZ), a toxin that produces hyperglycemia due to rapid and massive β cell death. After SZ-mediated elimination of existing IN+/PDX-1+ cells, a population of somatostatin (SOM)+/PDX-1+ cells, a cell type thought to represent an embryonic islet precursor cell, appeared in islets. The appearance of SOM+/PDX-1+ cells was followed in time by the differentiation to SOM+/IN+/PDX-1+cells. SOM+/PDX-1+ cells also appeared in islets of nonobese diabetic mice, a strain of mice in which β cell destruction is immune-mediated. Our findings establish the existence of PDX-1+ β cell precursors in the adult pancreas and indicate that their differentiation is induced by islet injury .
Oxford University Press