Quantitative analysis of prostaglandins in cell culture medium by high-resolution gas chromatography with electron-capture detection
ME Berens, SE Salmon, TP Davis - Journal of Chromatography B …, 1984 - Elsevier
Prostaglandins have been shown to be important modulators of haemostatis, immune
responses, and growth of normal and neoplastic cells. In order to investigate the cell origin
and metabolic profile of the endogenous prostaglandins in human tumours, a convenient
extraction and gas chromatographic method for measuring the various classes of
prostaglandins was developed. Infiltrating macrophages from human tumours were isolated
using adherence to plastic. Macrophage-enriched and macrophage-depleted cell …
responses, and growth of normal and neoplastic cells. In order to investigate the cell origin
and metabolic profile of the endogenous prostaglandins in human tumours, a convenient
extraction and gas chromatographic method for measuring the various classes of
prostaglandins was developed. Infiltrating macrophages from human tumours were isolated
using adherence to plastic. Macrophage-enriched and macrophage-depleted cell …
