Interleukin‐17 up‐regulation of nitric oxide production in human osteoarthritis cartilage
MG Attur, RN Patel, SB Abramson… - Arthritis & Rheumatism …, 1997 - Wiley Online Library
MG Attur, RN Patel, SB Abramson, AR Amin
Arthritis & Rheumatism: Official Journal of the American College …, 1997•Wiley Online LibraryObjective. To examine the effect of human interleukin‐17 (IL‐17) on nitric oxide (NO)
production in human osteoarthritis (OA) cartilage under ex vivo conditions. Methods. OA
cartilage from patients undergoing knee replacement surgery was used in explant assays to
assess the effect of IL‐17. NO production was measured by estimating the stable NO
metabolite, nitrite, in conditioned medium. Results. IL‐17 augmented the spontaneous
production of nitric oxide. This augmentation was sensitive to cycloheximide and pyrrolidine …
production in human osteoarthritis (OA) cartilage under ex vivo conditions. Methods. OA
cartilage from patients undergoing knee replacement surgery was used in explant assays to
assess the effect of IL‐17. NO production was measured by estimating the stable NO
metabolite, nitrite, in conditioned medium. Results. IL‐17 augmented the spontaneous
production of nitric oxide. This augmentation was sensitive to cycloheximide and pyrrolidine …
Abstract
Objective. To examine the effect of human interleukin‐17 (IL‐17) on nitric oxide (NO) production in human osteoarthritis (OA) cartilage under ex vivo conditions.
Methods. OA cartilage from patients undergoing knee replacement surgery was used in explant assays to assess the effect of IL‐17. NO production was measured by estimating the stable NO metabolite, nitrite, in conditioned medium.
Results. IL‐17 augmented the spontaneous production of nitric oxide. This augmentation was sensitive to cycloheximide and pyrrolidine dithiocarbamate, but not to dexamethasone or soluble IL‐1 receptor.
Conclusion. IL‐17 augments nitric oxide production in OA cartilage via nuclear factor KB activation, but independently of IL‐1β signaling.
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