[PDF][PDF] Flow-cytometric characterization of stimulation, free radical formation, peroxidase activity and phagocytosis of human granulocytes with 2, 7-dichlorofluorescein …
S Burow, G Valet - Eur J Cell Biol, 1987 - classimed.de
Model experiments with the nonfluorescent DCFH-DA cleav~ age product DCFH (2, 7—
dichlorofluoresein) showed that DCFH was quickly photo-oxidized to fluorescent DCF (2, 7-
dichlor0f'luo'rescein) by UV-light and to a lower degree by daylight DCFH even slowly
autooxidized in the dark 'As a consequence, DCFH-DA was dissolved and stored in organic
solvents in the dark to prevent spontaneous hydrolysis and autooxidation. All cellular assays
prior to the flow-cytometric measurements were stored and incubated strictly in the dark.
dichlorofluoresein) showed that DCFH was quickly photo-oxidized to fluorescent DCF (2, 7-
dichlor0f'luo'rescein) by UV-light and to a lower degree by daylight DCFH even slowly
autooxidized in the dark 'As a consequence, DCFH-DA was dissolved and stored in organic
solvents in the dark to prevent spontaneous hydrolysis and autooxidation. All cellular assays
prior to the flow-cytometric measurements were stored and incubated strictly in the dark.
