[HTML][HTML] Reversible inactivation of protein-tyrosine phosphatase 1B in A431 cells stimulated with epidermal growth factor
SR Lee, KS Kwon, SR Kim, SG Rhee - Journal of Biological Chemistry, 1998 - ASBMB
Stimulation of various cells with growth factors results in a transient increase in the
intracellular concentration of H 2 O 2 that is required for growth factor-induced protein
tyrosine phosphorylation. The effect of H 2 O 2 produced in response to epidermal growth
factor (EGF) on the activity of protein-tyrosine phosphatase 1B (PTP1B) was investigated in
A431 human epidermoid carcinoma cells. H 2 O 2 inactivated recombinant PTP1B in vitro by
oxidizing its catalytic site cysteine, most likely to sulfenic acid. The oxidized enzyme was …
intracellular concentration of H 2 O 2 that is required for growth factor-induced protein
tyrosine phosphorylation. The effect of H 2 O 2 produced in response to epidermal growth
factor (EGF) on the activity of protein-tyrosine phosphatase 1B (PTP1B) was investigated in
A431 human epidermoid carcinoma cells. H 2 O 2 inactivated recombinant PTP1B in vitro by
oxidizing its catalytic site cysteine, most likely to sulfenic acid. The oxidized enzyme was …
