Inhibition of hepatitis C virus (HCV)-RNA-dependent translation and replication of a chimeric HCV poliovirus using synthetic stabilized ribozymes
DG Macejak, KL Jensen, SF Jamison, K Domenico… - Hepatology, 2000 - journals.lww.com
Ribozymes are catalytic RNA molecules that can be designed to cleave specific RNA
sequences. To investigate the potential use of synthetic stabilized ribozymes for the
treatment of chronic hepatitis C virus (HCV) infection, we designed and synthesized
hammerhead ribozymes targeting 15 conserved sites in the 5′ untranslated region (UTR)
of HCV RNA. This region forms an internal ribosome entry site that allows for efficient
translation of the HCV polyprotein. The 15 synthetic ribozymes contained modified …
sequences. To investigate the potential use of synthetic stabilized ribozymes for the
treatment of chronic hepatitis C virus (HCV) infection, we designed and synthesized
hammerhead ribozymes targeting 15 conserved sites in the 5′ untranslated region (UTR)
of HCV RNA. This region forms an internal ribosome entry site that allows for efficient
translation of the HCV polyprotein. The 15 synthetic ribozymes contained modified …