Tumor localization of anti-CEA single-chain Fvs: improved targeting by non-covalent dimers
AM Wu, W Chen, A Raubitschek, LE Williams… - Immunotechnology, 1996 - Elsevier
Background: Genetic engineering can produce novel antibody fragments with improved
properties for applications such as tumor targeting in vivo. Objectives: To produce stable
monomeric (27 kDa) and dimeric (55 kDa) forms of a single-chain Fv (scFv) from the anti-
carcinoembryonic antigen (anti-CEA) antibody T84. 66, and assess the targeting and
biodistribution properties in an animal model. Study design: ScFv were constructed with
either a 28 or 14 amino acid connecting peptide and expressed by secretion from E. coli …
properties for applications such as tumor targeting in vivo. Objectives: To produce stable
monomeric (27 kDa) and dimeric (55 kDa) forms of a single-chain Fv (scFv) from the anti-
carcinoembryonic antigen (anti-CEA) antibody T84. 66, and assess the targeting and
biodistribution properties in an animal model. Study design: ScFv were constructed with
either a 28 or 14 amino acid connecting peptide and expressed by secretion from E. coli …